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<p><b>Microarrays - a Revolution in Life Science</b></p>
<p>It is widely believed that thousands of genes and their protein products function 
  in a complicated and orchestrated way that creates the mystery of life. However, 
  traditional methods in molecular biology (Northern Blot, RT-PCR, nuclease protection 
  assays) generally work on a "one gene in one experiment" basis, which means 
  that the "big" picture of gene function is hard to obtain.</p>
<p>A new technology, called DNA microarrays (gene chips), promises to monitor 
  the whole genome on a single chip so that researchers can have a better picture 
  of the interactions among thousands of genes simultaneously. This technology 
  allows high-throughput sequence recognition and gene expression analysis. The 
  high density of nucleic acid sequences can be spotted in a small area to which 
  labeled nucleic acid probes (radioactively- or fluorescently labeled) corresponding 
  to total cellular mRNA are hybridized. A sample gene expression pattern is obtained 
  by analyzing the signal at each spot and comparing it to the signal intensity 
  of the control sample. Using DNA microarrays, one can compare the expression 
  of multiple genes in different tissues, developmental stages, normal and diseased 
  states or cells subjected to different drugs.</p>
<p>The extraordinary power of DNA microarrays will have a strong impact on medicine 
  in the near future both in the molecular characterization of diseases and in 
  drug discovery and evaluation, and it is to be expected that quantitative applications 
  will soon spread through all fields of life sciences.</p>
<p align="center"><img src="p/OKrnd_ljudi_3.jpg" width="300" height="193" border=1></p>
1	dpavlin	1.1	<p><b>Microarrays - a Revolution in Life Science</b></p>
2			<p>It is widely believed that thousands of genes and their protein products function
3			in a complicated and orchestrated way that creates the mystery of life. However,
4			traditional methods in molecular biology (Northern Blot, RT-PCR, nuclease protection
5			assays) generally work on a "one gene in one experiment" basis, which means
6			that the "big" picture of gene function is hard to obtain.</p>
7			<p>A new technology, called DNA microarrays (gene chips), promises to monitor
8			the whole genome on a single chip so that researchers can have a better picture
9			of the interactions among thousands of genes simultaneously. This technology
10			allows high-throughput sequence recognition and gene expression analysis. The
11			high density of nucleic acid sequences can be spotted in a small area to which
12			labeled nucleic acid probes (radioactively- or fluorescently labeled) corresponding
13			to total cellular mRNA are hybridized. A sample gene expression pattern is obtained
14			by analyzing the signal at each spot and comparing it to the signal intensity
15			of the control sample. Using DNA microarrays, one can compare the expression
16			of multiple genes in different tissues, developmental stages, normal and diseased
17			states or cells subjected to different drugs.</p>
18			<p>The extraordinary power of DNA microarrays will have a strong impact on medicine
19			in the near future both in the molecular characterization of diseases and in
20			drug discovery and evaluation, and it is to be expected that quantitative applications
21			will soon spread through all fields of life sciences.</p>
22			<p align="center"><img src="p/OKrnd_ljudi_3.jpg" width="300" height="193" border=1></p>